Heparin lyase II CAS NO.149371-12-0

Heparinase II eaves heparan sulfate, and to a lesser extent heparin (relative activity about 2:1), at the 1-4 linkages between hexosamines and uronic …

Heparinase II eaves heparan sulfate, and to a lesser extent heparin (relative activity about 2:1), at the 1-4 linkages between hexosamines and uronic acid residues (both glucuronic and iduronic), yielding mainly disaccharides. Recombinant Heparinase II Flavobacterium hepar- inum; Host Cell: E. Coli. Property: l pH stability 7.0-8.0 l Optimum pH 7.5 l Thermal stability ≤25℃ (pH7.3, 20min) l Optimum temperature 35℃ l Molecular weight 84,100 l Isoelectric point 9.0 Specification l Specific activity >5.5 IU/mg. Enzyme activity definition One Heparinase II international unit (IU) is defined as the amount of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from porcine intestinal mucosal heparin per minute at 37oC and pH 7.3.